- 作者: Chih-Hung Chen, Li-Teh Chang, Wei-Chih Tung, Yung-Lung Chen, Chia-Lo Chang, Steve Leu, Cheuk-Kwan Sun, Tzu-Hsien Tsai, I-Ting Tsai, Hsueh-Wen Chang and Hon-Kan Yip
- 作者服務機構: Divisions of General Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: The roles of circulating endothelial progenitor cell (EPC) and mononuclear cell apoptosis
(MCA) in liver cirrhosis (LC) patients are unknown. Moreover, vascular endothelial growth
factor (VEGF) and stromal cell-derived factor (SDF)-1α are powerful endogenous substances
enhancing EPC migration into circulation. We assessed the level and function of EPCs
[CD31/CD34 (E1), KDR/CD34 (E2), CXCR4/CD34 (E3)], levels of MCA, VEGF and SDF-
1α in circulation of LC patients.
Methods: Blood sample was prospectively collected once for assessing EPC level and function, MCA,
and plasma levels of VEGF and SDF-1α using flow cytometry and enzyme-linked
immunosorbent assay (ELISA), respectively, in 78 LC patients and 25 age- and gendermatched
healthy controls.
Results: Number of EPCs (E1, E2, E3) was lower (all p < 0.0001), whereas SDF-1α level and MCA
were higher (p < 0.001) in study patients compared with healthy controls. Number of EPCs
(E2, E3) was higher but MCA was lower (all p < 0.05) in Child's class A compared with
Child's class B and C patients, although no difference in VEGF and SDF-1α levels were
noted among these patients. Chronic hepatitis B and esophageal varices bleeding were
independently, whereas chronic hepatitis C, elevated aspartate aminotransferase (AST), and
decompensated LC were inversely and independently correlated with circulating EPC level
(all p < 0.03). Additionally, angiogenesis and transwell migratory ability of EPCs were
reduced in LC patients than in controls (all p < 0.001). - 中文關鍵字: --
- 英文關鍵字: Liver cirrhosis, Circulating endothelial progenitor cells, Angiogenesis factors, Cellular apoptosis