- 作者: Roberta M. Magai Shivendra D. Shukla
- 作者服務機構: Deprtments of Pharmacology, University of Missouri School of Medicine, Columbia, Mo., USA
- 中文摘要: --
- 英文摘要: The metabolic fate of ethanol into the phospholipid poolof calf pulmonary artery endothelial cells was studied. [ C]-ethanol was incorporated into various endothelialcell phospholipids including phosphatidylethanol (PEth),which may represent a substantial fraction in microdo-mains of membrane phospholipids. The incorporationinto phospholipids was reduced in the presence of pyra-zole and cyanamide, inhibitors of ethanol metabolism.Wortmannin, the phosphatidylinositol 3-kinase inhibitor,increased[ C]-PEth formation.[ H]-acetate was also in-corporated into endothelial cell phospholipids but in adifferent pattern. Distribution of [ H]-acetate and[ C]-ethanol into the fatty acyl moiety versus the glycero-phosphoryl backbone of the phospholipids was also dif-ferent. Stimulation of the endothelial cells with ATPincreased [ H]-acetate incorporation into platelet-activat-ing factor (PAF) and ethanol decreased it. Ethanol expo-sure increased ATP-stimulated [ H]-acetate incorpora-tion into sphingomyelin. However, ATP had no effect onthe incorporation of[ C]-ethanol into any phospholip-ids. The results suggest that the two precursors contrib-ute to a separate acetate pool and that the sphingomy-elin cycle may be sensitized in ethanol-treated cells.Thus, metabolic conversions of ethanol into lipids andthe effect of ethanol on specific lipid mediators, e.g PAF,PEth and sphingomyelin, may be critical determinants inthe altered responses of the endothelium in alcoholism.
- 中文關鍵字: --
- 英文關鍵字: Phospholipids. Ethanol. Platelet-activating factor. Sphingomyelin. Phosphatidylethanol. Endothelial cells