第3卷‧第8期,
197508
, pp. 27-34
染色質之多二磷酸腺瞟呤核?核糖
- 作者:
林耀輝
- 作者服務機構:
Associate Research Fellow Institute of Botany; Academia Sinica
- 中文摘要:
An enzyme activity which uses NAD tosynthesize poly (ADP-Rib) has been detectedin nuclear preparations of all animal cellstested. Natural occurrence of poly (ADP-Rib)and poly (ADP-Rib) histones has been confir-med by in vivo experiments. No activity, how-ever, was found in prokaryotic organisms tes-ted. The author discovered that the crudechromatin of higher plants Oryza sativa andPhaseolus radirztus has NAD-incorporating sys-tem. Poly (ADP-Rib) polymerase binds firmlyto chromatin. It requires DNA for activity.The optimum pH is around 7.5. Its activity isinhibited strongly by nicotinamide and parach-loromercuribenzoate. Snake venom phosphodi-esterase is of use in determining the structureof poly (ADP-Rib). A glycohydrolase foundin chromatin may be responsible for degrada-tion of the polymer in vivo. Poly (ADP-Rib)may playa role in maintaining the structureof chromatin and regulating the function ofchromatin such as DNA synthesis.
- 英文摘要:
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- 中文關鍵字:
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- 英文關鍵字:
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