- 作者: Jianquan Zhao, Tiewei Lv, Junjun Quan, Weian Zhao, Jing Song, Zhuolin Li, Han Lei, Wei Huang and Longke Ran
- 作者服務機構: 1. Department of Cardiology, Bayannaoer City Hospital, 35 Xinhua District, Bayannaoer 015000, Inner Mongolia, China 2. Department of Vascular Cardiology, the First Affiliated Hospital of Chongqing, Medical University, Chongqing, China 3, Department of Bioinformatics, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China
- 中文摘要:
- 英文摘要:
Background
Identify genes probably associated with chronic heart failure and predict potential target genes for dilated cardiomyopathy using bioinformatics analyses.
Methods
Gene expression profiles (series number GSE3585 and GSE42955) of cardiomyopathy patients and healthy controls were downloaded from the Expression Omnibus Gene (GEO) database. Differential expression of genes (DEGS) between the two groups of total 14 cardiomyopathy patients and 10 healthy controls were subsequently identified by limma package of R. Database for Annotation, Visualization, and Integrated Discovery (DAVID Tool), which is an analysis of enriched biological processes. Search Tool for the Retrieval Interacting Genes (STRING) was used as well for the analysis of protein-protein interaction network (PPI). Prediction of the potential drugs was suggested based on the preliminarily identified genes using Connectivity Map (CMap).
Results
Eighty-nine DEGs were identified (57 up-regulated and 32 down-regulated). The most enrichment Gene Ontology (GO) terms (P < 0.05) contain genes involved in extracellular matrix (ECM) and biological adhesion signal pathways (P < 0.05, ES > 1.5) such as ECM-receptors, focal adhesion and transforming growth factor beta (TGF-β), etc. Fifty-one differentially expressed genes were found to encode interacting proteins. Eleven key genes along with related transcription factors were identified including CTGF, POSTN, CORIN, FIGF, etc.
Conclusion
Bioinformatics-based analyses reveal the targeted genes probably associated with cardiomyopathy, which provide clues for pharmacological therapies aiming at the targets. - 中文關鍵字:
- 英文關鍵字: Dilated cardiomyopathy, Bioinformatics, Microarray, Heart failure