• 作者： 張珍田; 鄭淑文; 宋賢一; 蘇仲卿
• 作者服務機構： 私立靜宜學院食品營養學系; 國立臺灣大學農業化學系
• 中文摘要： 自經由適當自家分解作用的豬胰臟，製備了含高活性蛋白?、脂肪?及澱粉?之胰臟素。自每一公斤之豬胰臟約可
  製取一百克左右之胰臟素。
  經由微鹼性液抽提，親和吸著法除去蛋白?，第一次硫酸銨劃分， Sephacryl S-200 膠體過濾以及第二次硫酸銨劃
  分等系列步驟，由所製備之胰臟素可得純化之脂肪?。經此等步驟可提高酵素純度14倍並獲得33％之活性收率。此純化
  之脂肪?，以聚丙烯醯胺膠體電泳法鑑定時顯示其品質並非均一而尚含有數種其他蛋白質。
  冬
• 英文摘要： Pancreatin containing high activities of proteolytic enzymes, amylase and lipase was prepared fromoptimally autolyzed hog pancreas. About one hundred grams of pancreatin were obtained from one kilo-gram of hog pancreas. Lipase was purified from the pancreatin preparation through steps of mild alkaline solution extrac-tion, removing proteolytic enzymes by affinity adsorption, first ammonium sulfate fractionation, SephacrylS-200 gel filtration, and secondary ammonium sulfate fractionation. By these steps, the purity of the en-zyme increased 14 fold and the recovery of the enzyme activity was 33%. The purified lipase was nothomogeneous and contained several contaminating proteins when examined by disc polyacrylamide gelelectrophoresis.
• 中文關鍵字： hog pancreas; pancreatin; lipase
• 英文關鍵字： --