- 作者: Yi-Ting Huang, Jia-Teh Liao, Li-Chen Yen, Yung-Kun Chang, Yi-Ling Lin, Ching-Len Liao
- 作者服務機構: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei , Taiwan, Republic of China (ROC)
- 中文摘要: --
- 英文摘要:
Background:
To construct safer recombinant flavivirus vaccine, we exploited Japanese encephalitis virus (JEV) replicon-based platform to generate single-round infectious particles (SRIPs) that expressed heterologous neutralizing epitope SP70 derived from enterovirus-71 (EV71). Such pseudo-infectious virus particles, named SRIP-SP70, although are not genuine viable viruses, closely mimic live virus infection to elicit immune responses within one round of viral life cycle.
Results:
We found that, besides gaining of full protection to thwart JEV lethal challenge, female outbred ICR mice, when were immunized with SRIP-SP70 by prime-boost protocol, could not only induce SP70-specific and IgG2a predominant antibodies but also provide their newborns certain degree of protection against EV71 lethal challenge.
Conclusions:
Our results therefore exemplify that this vaccination strategy could indeed confer an immunized host a dual protective immunity against subsequent lethal challenge from JEV or EV71. - 中文關鍵字: --
- 英文關鍵字: Japanese encephalitis virus (JEV); Replicons; Single-round infectious particles (SRIPs); RNA vaccine; Enterovirus-71 (EV71); Neutralizing epitope; SP70; Dual protective immunity