- 作者: Jia-Horng Kao; Pei-Jer Chen ; Ming-Yang Lai; Pei-Ming Yang; Jin-Chuan Sheu; The-Hong Wang; Ding-Shinn Chen
- 作者服務機構: Department of Internal Medicine, Graduate Institute of Clinical Medicine, and Hepatitis Research Center, National Taiwan University College; of Medicine and; the University Hospital, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: The nucleotide sequences of the putative envelope region (E1) and the junc- tion between the E1 and envelope 2/nonstructural 1 (E2/NS1) region of the hepatitis C virus (HCV) genome are divergent among different genotypes. To characterize them, we introduced a set of nested primers that are conserved among four different genotypes (types I-IV) of HCV for polymerase chain reaction (PCR) amplification. The amplified products include the variable full-length E 1 region, and the 5' end of the E2/NS 1 region, the so-called hyper- variable region-1 (HVR-1). Of 53 patients with histologically confirmed chronic liver disease and HCV viremia, type II virus was the most dominant strain as detected by the PCR genotyping method and the envelope region could be amplified in more than half of them irrespective of their genotypes. The specificity was confirmed by subsequent nucleotide sequence analysis. The positivity of envelope region PCR was not correlated with histologic diag- nosis and hepatitis activities in these patients. Our results suggest that the nested primers can amplify the variable E1 and hypervariable 5' end of E2/ NS1 of the HCV genome with moderate efficiency, and thus will be useful in future studies of HCV infections.
- 中文關鍵字: --
- 英文關鍵字: HCV RNA; PCR; In vitro direct sequencing; HCV genotypes; Hypervariable region