• 作者： Chou, C. P.; Tseng, J. H.
• 中文摘要： 本文主要探討不同糖類添加對Penicillin acylase之生產與包涵體形成之效應。
• 英文摘要： The effect of carbon supplementation on penicillin acylase (PAC) production and inclusion body formation was investigated. The host effect was also examined by comparing culture performances using HB 101 or its derived mutant, MD.DELTA.P7, as the expression host. Among several carbons tested, glycerol was found to be optimum for PAC production. PAC activity was increased and the amount of inclusion bodies was reduced upon glycerol supplementation. The effect of carbon source on inclusion body formation appears to be strain-dependent. With the optimization on the culture parameters (i.e ., host and carbon effects), volumetric PAC activity could be significantly improved at more than fourfold that of the control experiment. This information is useful for developing expression strategies for optimization of PAC production.
• 中文關鍵字： 盤尼西林化; 包涵體; 基因表現; 碳源
• 英文關鍵字： Penicillin Acylase; Inclusion Body; Gene Expression; Carbon Source