- 作者: Lee-Wei Chen; Wei-Jung Chang; Pei-Hsuan Chen; Ching-Mei Hsu
- 作者服務機構: Institute of Emergency and Critical Care Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background : Major burn is associated with decreased gut barrier function and increased bacterial translocation (BT). This study is to
investigate whether commensal microflora induce host defense and decrease BT in burn mice.
Methods : First, we treated Wild type (WT) mice with antibiotics in drinking water for 4 weeks to deplete gut commensal
microflora. At week 3, drinking water was supplemented with lipopolysaccharide (LPS); a ligand for TLR4, to trigger
TLRs in gut. The intestinal permeability, glutathione level, NF-κB DNA-binding activity, TLR4 expression of intestinal
mucosa, BT to mesenteric lymph nodes (MLNs), and bacterial killing activity of peritoneal cells were measured after
thermal injury. Second, lung of animals were harvested for MPO activity and TNFα mRNA expression assay. Third,
WT animals were treated with oral antibiotics with or without LPS supplement after burn. At 48 hr after burn, TLR4
expression of intestinal mucosa and bacterial killing activity of cells were examined. Finally, bacterial killing activity
and BT to MLNs after thermal injury in C3H/HeJ (TLR4 mutant) mice were measured.
Results : Burn induced BT to MLNs in WT mice. Commensal depletion decreased TLR4 expression as well as NF-κB activation
of intestine, myeloperoxidase (MPO) activity as well as TNFα expression of lung, and bacterial killing activity of
peritoneal cells. Oral LPS supplement markedly reduced 81% of burn-induced BT and increased TLR4 expression,
MPO activity of lung, as well as bacterial killing activity of peritoneal cells. LPS supplement did not change BT or
bacterial killing activity in C3H/HeJ mice.
Conclusions : Collectively, commensal microflora induce TLR4 expression of intestine and bacterial killing activity of inflammatory
cells in burn. TLR4 ligand increases bacterial killing activity and decreases burn-induced BT. Taken together with the
abolition of LPS effect in TLR4 mutant mice, we conclude that commensal microflora induce host defense and decrease
bacterial translocation in burn mice through toll-like receptor 4. - 中文關鍵字: --
- 英文關鍵字: --