- 作者: 葉東柏; 蘇仲卿
- 作者服務機構: 國立臺灣大學農業化學系; 中央研究院生物化研究所
- 中文摘要:
省產粗製尿激?(比活性均為10,000國際單位/毫克蛋白質)可簡單的經過膠體過濾層析及親和性層析法分離出重
型尿激?,比活性均為90,000國際單位∕毫克蛋白質,其純度經膠體電泳法證明為均質物。
由純化研究顯示欲單離重型尿激?,於膠體過濾層析法操作中應使用離子強度較高的緩衝液。否則,重型尿激?會
發生解離或分解而出現分子量較低的衍生物,並有聚合現象。
此外,氨基酸與醣質組成,及勝?指模圖亦經分析並與輕型尿激?的比較以瞭解其相關性。 - 英文摘要: The large-form urokinase (L-UK), which has been demonstrated to be more effective in treatingthrombosis, was isolated from a crude preparation of local source to electrophoretical homogeneity simplyby gel filtration and affinity chromatography. In the isolation of L-UK, a buffer solution of higher ionic strengh (such as 0.1 M phosphate buffercontaining 0.1 M or higher NaCI) must be used for eluting the enzyme from a Sephadex gel column. Other-wise, the L-UK may dissociate or degrade to forms of lower molecular weight (e.g. S-UK) with simultaneouspolymerization. This is in our opinion, one of the important reasons why variable forms of UK in variousratio were reported previously. In the assay of urokinase activity, the conventional fibrin plate method was modified to be a morereproducible and convbnient procedure by punching holes for sample application on an agar-containingfibrin plate. The amino acid as well as sugar compositions and tryptic peptide maps of L-UK were assessed andcompared with those of small-form urokinase (S-UK) from Mochida (Japan). It was found that there weregreat similarities among these enzyme proteins and the homology of L-UK and S-UK in primary structurewas proposed.
- 中文關鍵字: --
- 英文關鍵字: --