- 作者: Yuanbin Liu; David R Schubert
- 作者服務機構: Cellular Neurobiology Laboratory, The Salk Institute for Biological Studies, California, USA
- 中文摘要: --
- 英文摘要:
Background: Reactive oxygen species (ROS) play an important role in aging and age-related
diseases such as Parkinson’s disease and Alzheimer’s disease. Much of the ROS production
under conditions of toxic stress is from mitochondria, and multiple antioxidants prevent ROS
accumulation. The aim of this study is to examine the specificity of the interaction between the
antioxidants and ROS production in stressed cells.
Methods: Using fluorescent dyes for ROS detection and mitochondrial inhibitors of known
specificities, we studied ROS production under three conditions where ROS are produced by
mitochondria: oxidative glutamate toxicity, state IV respiration induced by oligomycin, and tumor
necrosis factor-induced cell death.
Results: We demonstrated that there are at least four mitochondrial ROS-generating sites in
cells, including the flavin mononucleotide (FMN) group of complex I and the three ubiquinonebinding
sites in complexes I, II and III. ROS production from these sites is modulated in an
insult-specific manner and the sites are differentially accessible to common antioxidants.
Conclusions: The inhibition of ROS accumulation by different antioxidants is specific to the site
of ROS generation as well as the antioxidant. This information should be useful for devising
new interventions to delay aging or treat ROS-related diseases. - 中文關鍵字: --
- 英文關鍵字: --