- 作者: Yi-Ling Yang; Kun-Lun Huang; Huey-Ling Liou; Hsing I. Chen
- 作者服務機構: Institute of Biotechnology, National Chia-Yi University,Chiayi, Taiwan; Institute of Undersea and Hyperbaric Medicine,National Defense Medical Center, Taipei, Taiwan; Department of Nursing, Taipei Veteran General Hospital, Taipei,Taiwan
- 中文摘要: --
- 英文摘要: Phorbol myristate acetate (PMA) causes acutelung injury (ALI). The present study was designed to elucidatethe role of nitric oxide (NO), inducible NO synthase(iNOS), neutrophil elastase (NE) and other mediators in theALI caused by PMA. In isolated rat’s lungs, PMA at variousdoses (1, 2 and 4 lg/g lung weight) was added into the lungperfusate. Vehicle group received dimethyl sulfoxide (thesolvent for PMA) 100 lg/g. We measured the lung weightchanges, pulmonary arterial pressure, capillary filtrationcoefficient, exhaled NO, protein concentration in bronchoalveolarlavage (PCBAL) and Evan blue dye leakage.Nitrate/nitrite, methyl guanidine, proinflammatory cytokines,NE and myeloperoxidase (MPO) in lung perfusatewere determined. Histopathological examination was performed.We detected the iNOS mRNA expression in lungtissue. PMA caused dose-dependent increases in variablesfor lung changes, and nitrate/nitrite, methyl guanidine, proinflammatorycytokines, NE andMPOin lung perfusate. Thepathology was characterized by alveolar hemorrhagic edemawith inflammatory cell infiltration. Scanning electronmicroscopy revealed endothelial damage. PMA upregulatedthe expression of iNOS mRNA. Our results suggest thatneutrophil activation by PMA causes release of NE, upregulationof iNOS and a series of inflammatory responsesleading to endothelial damage and ALI.
- 中文關鍵字: --
- 英文關鍵字: Acute lung injury; Phorbol myristate acetate; Inducible nitric oxide synthase; Neutrophil elastase; Myeloperoxidase; Proinflammatory cytokines