- 作者: M.C. Audy; P. Vacher; A.M. Vacher; B. Dufy
- 作者服務機構: Laboratoire de Neurophysiologie CNRS URA 1200, Universite de Bordeaux II, France
- 中文摘要: --
- 英文摘要: We investigated the effects of prolactin (PRL) on the protein kinase C (PKC) activity in Chinese hamster ovary (CHO-E32) cells stably transfected with rabbit mammary gland PRL receptor cDNA. These cells express a functional long form of PRL-R. A 10-min to 2-hour treatment with 5 nM PRL resulted in the translocation of PKC activity from the cytosol to the membrane. Longer treatment (10-24 h) with the same concentration of PRL decreased the PKC activity in both particulate and cytoplasmic fractions. The PRL effect was dose dependent: maximal action was obtained with 1-10 nM. The PRL induced activation of PKC was blocked by 20 nM staurosporine, a PKC inhib itor. Two inhibitors of tyrosine kinase, herbimycin A (1.75 μM) and genistein (100 μM), had no effect on PRL-induced activation of PKC.
- 中文關鍵字: --
- 英文關鍵字: Chinese hamster ovary cells Phorbol myristate acetate Prolactin Protein kinase C Tyrosine kinase