- 作者: Wei-Teing Chen Wen-Hsin Huang David Wang Fu-Chiu Yu Ying-Chih Chi Jen- Chine Wu Kerry Wu Wann-Cherng Perng Chin-Pyng Wu Horng-Chin Yan
- 作者服務機構: Tri-Service General Hospital, School of Pharmacy, Graduate Institute of LIfe Sciences, National Defense Medical Center, and Fu-Jen Catholic University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Adenosine triphosphate (ATP)-MgCl2 attenuates isch-emia-reperfusion(I-R)-induced lung injury in rats. A pre-vious study indirectly suggests that Mg2+-dependentecto-ATPases on the surface of leukocytes are responsi-ble for the hydrolysis of ATP-MgCl2 to adenosine, whichthen contributes to the protective effect of ATP-MgCl2.This study investigated the role of leukocytes in I-R injuryand the protective effect of ATP-MgCl2 in our buffer-per-fused isolated rat lung model.After isolating the lungblood flow of adult male Sprague-Dawley rats, the lungswere perfused through the pulmonary artery cannulawith a physiologic salt solution containing human serumalbumin.The protective effect of ATP-MgCl2 pretreat-ment with or without leukocytes was investigated.Capil-lary permeability(Kfc),lung weight gain(LWG),lung wetweight/body weight ratio(LW/BW),lung lavage proteinconcentration(LPC)and pulmonary artery pressure(PAP) were measured.I-R produced a significant in-crease in Kfc, LWG,LW/BW, LPC, and PAP. The increasesin these indices were significantly attenuated by pre-treatment with ATP-MgCl2(1 x 10-6 M) together withleukocytes(2.9 x 106/ml in the perfusate)but not withATP-MgCl2 alone.Our data suggest that I-R-inducedacute lung injury is not dependent on circulating leuko-cytes. Pretreatment with ATP-MgCl2 plus leukocytes butnot ATP-MgCl2 alone had protective effects against I-Rlung injury. Whether these findings occur in vivo couldnot be determined in this study. In our isolated lung redblood cell-free perfusate system,the protective effect ofATP-MgCl2 requires the presence of leukocytes.
- 中文關鍵字: --
- 英文關鍵字: Adenosine. Adenosine triphosphate. Acute lung injury. Leukocytes