• 作者： Jing-You Guo, I-Ju Liu, Hsiu-Ting Lin, Mei-Jung Wang, Yu-Ling Chang, Shin-Chang Lin, Mei-Ying Liao, Wei-Chia Hsu, Yi-Ling Lin, James C. Liao and Han-Chung Wu
• 作者服務機構： 1.Institute of Biologic Chemistry, Academia Sinica, Taipei, Taiwan 2.Institute of Cellular and Organismic Biology, Academia Sinica, No. 128, Academia Road, Section 2, Nangang, Taipei, 11529, Taiwan 3.Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
• 中文摘要：
• 英文摘要：
  Background
  Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading to more than 3.3 million deaths. Identification of immunological epitopes on the virus would be highly useful for the development of diagnostic tools and vaccines that will be critical to limiting further spread of COVID-19.
  
  Methods
  To find disease-specific B-cell epitopes that correspond to or mimic natural epitopes, we used phage display technology to determine the targets of specific antibodies present in the sera of immune-responsive COVID-19 patients. Enzyme-linked immunosorbent assays were further applied to assess competitive antibody binding and serological detection. VaxiJen, BepiPred-2.0 and DiscoTope 2.0 were utilized for B-cell epitope prediction. PyMOL was used for protein structural analysis.
  
  Results
  36 enriched peptides were identified by biopanning with antibodies from two COVID-19 patients; the peptides 4 motifs with consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. The putative epitopes and hit peptides were then synthesized for validation by competitive antibody binding and serological detection.
  
  Conclusions
  The identified B-cell epitopes on SARS-CoV-2 may aid investigations into COVID-19 pathogenesis and facilitate the development of epitope-based serological diagnostics and vaccines.
• 中文關鍵字：
• 英文關鍵字： COVID-19, SARS-CoV-2, Phage-displayed peptides, B-cell epitope, Diagnostic tool, Serological detection