- 作者: Koko Murakami; Yen Li
- 作者服務機構: Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Md., USA
- 中文摘要: --
- 英文摘要: The SF1 element (nucleotide -135 to -131) in the U3 region of the simian immunodeficiency virus (SIV) 5' to the NF-κB binding site plays a role in basal expression of SIV [Winandy et al., J Virol 66:5216-5223, 1992]. Using the electrophoretic mobility shift assay, we have characterized a specific SF1 binding factor (sf1) in the nuclear fractions of transformed T cells as well as monocytic and macrophage leukemic cells. A strong SF1 binding activity was demonstrated in all cell types. The SF1 binding activity was unaffected by treatment with PMA, PHA, or Ca2+ ionophore. The HIV-1 long terminal repeat (LTR) 5' to the NF-κB site contained a homologous element which showed a specific binding complex of different mobility. The sf1 interacting with SIV LTR was composed of multiple proteins judged from UV crosslinking, some of which might be related to Jun/Fos proteins.
- 中文關鍵字: --
- 英文關鍵字: AIDS ; Retrovirus ; Transcription factor ; Affinity chromatography ; Gel mobility shift assay ; UV crosslinking