• 作者： 劉杭生; 石光興; 陳正繹; 趙壯飛
• 作者服務機構： 三軍總醫院婦產部; 國防醫學院生物及解剖學科; 三軍總醫院臨床醫學研究部細胞生物研究室
• 中文摘要： 雌二醇，黃體素或合成黃體素乙酸均可誘導哺乳類子宮中 17-β 氫氧基類固醇去氫?（17-β-HSD）。本實驗
  為進一步瞭解雌性素對子宮中 17-β-HSD 之誘導作用是經由及接受器接合後所產生之反應，因此利用子宮切除
  之未成熟鼠為材料，再分別腹腔注射或二乙基合成雌性素（DES）。就接受器而言，DES為之協同化合物
  ，但非 17-β-HSD ?受質。經連續注射第1, 14, 28天後之鼠分別測量子宮重量及觀察子宮內膜上皮細胞變化，以做為評
  估子宮對雌性素反應之指標。同時利用細胞化學方法及薄層層析術測定 17-β-HSD 之變化。結果顯示及 DES 處理
  之鼠其子宮重量及內膜上皮均呈明顯之改變，尤其在 14 天及 28 天組中最明顯。同時 17-β-HSD 在組中增加約兩
  倍，而 DES 組中增加四倍。結果各組中子宮重量與 17-β-HSD 之增加成〝負線性相關〞，而在 DES 及對照組中
  則無任何相關性。由以上結果可說明雌性素對 17-β-HSD 在鼠子宮中之誘導作用是經由與接受器接合後活化 17-β-
  HSD 基因。因此我們相信 17-β-HSD 基因在子宮中為另一個雌性素反應基因。a
• 英文摘要： Estradiol , progesterone or medroxyprogesterone acetate can induce biosynthesis of the 17-β-hydroxysteroid dehydrogenase (17-β-HSD) in the mammalian uterus. For further understanding the17-β-HSD induction which may be mediated by the conjugation of the to its receptor, prematureovariectomized rats were treated with . or with a synthetic steroid, diethylstilbestrol (DES), an agonistfor the receptor but not a substrate for 7-β-HSD. Histological observation and uterus weight wereexamined as parameters to evaluate uterine response to those hormones at different durations of treatment.The 17-β-HSD in ovariectomized rat uterus of each group was also examined by histochemical and bioche-mical assays. The results showed that the 17-β-HSD activity in the uterus can be induced by or DES,after daily treatment for 1, 14 and 28 days, but much higher in DES treated animals. The uterus weightdemonstrated a "negative linear correlation”to the enzyme activity in all treated groups, but not inDES or control rats. Accordingly, it was indicated that the 17-β-HSD induction was regulated by conjuga-tion of or DES to its receptor. Therefore, we believe that the 17-β-HSD gene in the rat uterus is anotherestrogen responsive gene.
• 中文關鍵字： diethystilbestrol; estradiol; uterus; 17-β-hydroxysteroid dehydrogenase; ovariectomized rat
• 英文關鍵字： --