- 作者: 王淑慧 ; 高心怡 ; 陳水田 等
- 作者服務機構: 中央研究院生物化學研究所
- 中文摘要: Racemic tert-leucine can be completely resolved by ligand exchange chromatography (LEC) using a Chiralpak WH column. The retention time of the tert-leucine was decreased by increasing concentration of Cu/sup 2+/, or decreasing pH of the eluent whereas it was unaffected by varying the temperature of eluent. Optimal conditions for complete separation of the D,L-tert-leucine were found to be(Cu/sup 2+/, 5.0mM, pH 5.5, 25.degree.C). The enantiomeric excess of synthetic tert-leucine, a chiral auxiliary reagent, was determined.
- 英文摘要: --
- 中文關鍵字: Chromatography; Leucine; Enantiomer
- 英文關鍵字: 層析法;亮氨酸;對映異構體