• 作者： Vie Cheng
• 作者服務機構： Department of Biology Tunghai University Taichung; Taiwan
• 中文摘要： 本研究以老鼠腎臟為材料，經活體滴定法固定後，利用電子顯微鏡，及形態定量統計法探討蛋白質穿越淋巴管壁之
  機制。淋巴管壁細胞內飲胞之體積，數量密度及其在細胞中分佈之情形以統計分析結果。淋巴管壁細胞接合之形態，細
  胞接合間隙之寬度，距離，亦加以記錄。實驗結果顯示細胞內飲胞之體積密度，在注射生理鹽水組為 0.024 。注射0.2
  mg/g體重蛋白素之老鼠，此值為 0.029 (p＜0.05)，注射0.4 mg/g體重蛋白素之處理組，提高為0.033 (p＜0.01)。在
  0.8 mg/g體重注射之處理組，此值降為0.022。飲胞數量密度在四組分析結果，則分別自食鹽注射組之27個/ 增加
  到0.2 mg/g體重蛋白素注射組之35個 / (p＜0.05)，經0.4 mg/g體重，蛋白素注射之處理組，此值增為44個 /
  (p＜0.01)，而在0.8 mg/g體重蛋白素之注射組?減為27個 / 。在細胞內飲胞之分佈上，各組平均有28%-38%面
  向淋巴管腔，有7%-16%面向組織間隙，其餘飲胞游離在細胞質中。以細胞接合間隙而言，在注射0.8 mg/g體重蛋白
  素後，開啟式接合之數目及間隙之距離顯著增加。
  s
• 英文摘要： The pathways involved in protein transport across the lymphatic endothelium of the rat renal cortexafter in vivo drip fixation were studied ultrastructurally. Both qualitative and quantitative analyses weremade on tissues from physiological saline-injected rats and on tissues from rats injected intravenously withrat albumin (0.2 mg/g body wt.,0.4 mg/g body wt. or 0.8 mg/g body wt.) The volume density values ofintracytoplasmic vesicles were as follows：(1) saline-injected rats: 0.024,(2) 0.2 mg/g body wt. albumin-injected rats: 0.029 (p＜0.05), (3) 0.4 mg/g body wt. albumin-injected rats increased to 0.033 (p＜0.01), (4) 0.8 mg/g body wt. albumin-injected rats had a value of 0.022. The numerical density of intracytoplasm-ic vesicles increased from 27/ after saline injection to 35/ (p＜0.05)after 0.2 mg/g body wt. albu-min injection. When rats were injected with 0.4 mg/g body wt. albumin, the numerical density was 44/(P＜0.01, in comparison with saline-injected rats) but this value decreased to 27/ in rats injected with0.8 mg/g body wt. albumin. In the four groups, the range of vesicles was 28-38% opening into the lym-phatic lumen and 7-16% of vesicles opening into the interstitial space. The remaining vesicles were free inthe endothelial cotyplasm. There were more open junctions and wider cell junctions in the 0.8 mg/g bodywt. albumin-injected group. It is concluded that normally albumin molecules are transported into the lymphatic capillaries byintracytoplasmic vesicles or through the normal interstitial space between endothelial cells. Vesiculartransport plays a more significant role in translymphatic protein movement when plasma albumin concen-tration is increased moderately. At higher plasma protein concentrations, however, the increased numberof open junctions or open regions is considered to play a more important role in lymph formation.
• 中文關鍵字： albumin; transport; lymphatics; ultrastructure
• 英文關鍵字： --