- 作者: 林山陽 ; 吳文修
- 作者服務機構: 榮民總醫院醫學研究部
- 中文摘要: A rapid and comprehensive sample preparation method used to extract adriamycin from plasma has been investigated. The samples were passed through an Adsorbex extraction column with an elution solvent. The eluate was directly analyzed by reversed- phase high performance liquid chromatography (HPLC) with fluoresence detector. This solid/liquid extraction method is simpler than the conventional liquid/liquid extraction method. Different elution solvents were used to extract the adriamycin from samples with and without serum. The mobile phase was found to be the optimal elution solvent: 5 mM orthophosphoric acid 62.5% in methanol: acetonitrile: isopropanol=15:15:7.5, pH 3.2. This method has the merits of better recovery (98%), higher reproducibility, and requires shorter time and consumes less solvent.
- 英文摘要: --
- 中文關鍵字: Adriamycin; Adsorbex Extraction Column; Hplc; Sample Preparation; Plasma; Analysis
- 英文關鍵字: 血漿;萃取管柱;高效液相層析;樣品配製;分析