- 作者: Shang-Hua Yang; Mei-Lan Liu; Chih-Feng Tien; Shih-Jie Chou; Ruey-Yi Chang
- 作者服務機構: Institute of Biotechnology and Department of Life Science, National Dong Hwa University, Taiwan, R. O. C.
- 中文摘要: --
- 英文摘要:
Replication of the Japanese encephalitis virus (JEV) genome depends on host factors
for successfully completing their life cycles; to do this, host factors have been recruited
and/or relocated to the site of viral replication. Glyceraldehyde-3-phosphate
dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral
RNA-dependent RNA polymerase (NS5) in JEV-infected cells. Subcellular
fractionation further indicated that GAPDH remained relatively constant in the cytosol,
while increasing at 12 to 24 hours postinfection (hpi) and decreasing at 36 hpi in the
nuclear fraction of infected cells. In contrast, the redistribution patterns of GAPDH
were not observed in the uninfected cells. Co-immunoprecipitation of GAPDH and JEV
NS5 protein revealed no direct protein-protein interaction; instead, GAPDH binds to the
3’ termini of plus- and minus-strand RNAs of JEV by electrophoretic mobility shift
assays. Accordingly, GAPDH binds to the minus strand more efficiently than to the
plus strand of JEV RNAs. This study highlights the findings that infection of JEV
changes subcellular localization of GAPDH suggesting that this metabolic enzyme may
play a role in JEV replication. - 中文關鍵字: --
- 英文關鍵字: --