第8卷‧第1期,
198001
, pp. 21-27
正常胎盤及滋養層細胞腫瘤所分泌的性腺激素之研究
- 作者：
歐陽培銓; 林榮耀; 潘瓊君; 陳明宗
- 作者服務機構：
國立臺灣大學醫學院婦產科生化科
- 中文摘要：
取正常妊娠婦人葡萄胎或絨毛癌病人的尿液，先由 Kaolin-Acetone 的方法初步取得人類胎盤性腺激素 (HCG)，再進一步經色層管柱分析決獲得較純的 HCG。此等色層管柱分別有Column Chromatography Sephadex G200, DEAB Sephadex A-50及Sephadex G100。 純化所得的 HCG 經SDS-凝膠電泳與不連續凝膠電泳，顯示出 HCG 為具有二個次單元體，其分子量分別為 26,000 及 35,000。 由葡萄胎患者的尿液中純化出來的 HCG 經胺基酸分析，所得的結果脯胺基特別的多，而沒有色胺酸。 將純化的 HCG 與 Complete Freund's Adjuvant 混合注射到雄性成熟兔子的皮下，數週後抽血取 HCG 抗體並加以純化，最後以免疫擴散試驗來測 HCG 體的 Titer，本實驗得到的抗體 Titer 只稀釋到8倍而已，相當的低，其原因現在研探中。
- 英文摘要：
The crude human chorionic gonadotropinwas extracted from the urine of patients withhydatidiform mole or choriocarcinoma byKaolin-acetone method of Albert. The extractwas dialysed against distilled water, and thenlyophillized and stored at 4 C for further use. HCG was further purified by three stepsinvolved, column chromatography on SephadexG200, DEAB Sephadex A-50 and SephadexG100. The purified HCG shown on SDS-gelelectrophoresis was dissociated into two subunitsin 4M urea acrylamide gel electrophoresis.The apparent molecular weights of thesesubunits were estimated to be 26,000 and 35,000. The purified HCG was mixed with completeFreund's adjuvant and injected subcutaneouslyinto the rabbits to raise antibody. The activefractions were obtained and．the titer wasestimated. However, the titer was not sufficientlyhigh for the clinical use. The reason of thelow titer is now placed under investigation.
- 中文關鍵字：
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- 英文關鍵字：
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