• 作者： 章淑貞; 陳甘霖; 柴惠珍; 楊永孚
• 作者服務機構： 三軍總醫院醫學研究部﹑婦產部; 國防醫學院藥學系;
• 中文摘要： 雌三醇－酵素複合體（Estriol-enzyme conjugate）之製備，為雌三醇之酵素免疫分析中之必
  要步驟，本報告將?述雌三醇－酵素複合體之製備、純化、醇素的總活性、免疫活性之測定，及
  利用酵素免疫分析法來測定孕婦血清檢品中雌三醇之含量，
  從21位妊娠36～40週之正常孕婦，抽取其血液，以本實驗室所建立之酵素免疫分析法，測
  定血清游離型雌三醇（Unconjugated estriol）之含量，將所得之變動範圍與國內外學者所得之結　
  果做一比較。
• 英文摘要： Horseradish peroxidase (HRP) was coval-ently coupled to estriol-6-(O-carboxy methyl)oxime. The estriol enzyme conjugate was usedas marker in the development of a enzyme im-munoassay for the estriol. The assay dependson the competitive binding of estriol andestriol-HRP conjugate to the anti-estriol-3-OCH2COOH BSA serum. The free and boundhormone were separated by precipitation withpolyethylene glycol. The serum concentration of unconjugatedestriol was measured by enzyme immunoassayin 21 normal pregnancies from 36-41 weeks ofgastation. The mean value for serum estrioland limits of normal variation have been definedand compared with other investigators usingradioimmunoassay (RIA) or competitive proteinbinding (CPB).
• 中文關鍵字： --
• 英文關鍵字： --