• 作者： Chao, Hsin-Peng; Lee, Wen-Chien
• 中文摘要： 酵素以麩蛋白包埋固定形成薄膜, 將所得薄膜與pH電極結合可得一盤尼西林電極。使用麩蛋白有許多好處, 例如生物可分解性、對環境無害、便宜、以及取得容易。在下同緩衝溶液, 不同盤尼西林濃度之下, 或是不同薄膜厚度時, 這個盤尼西林電極的應答皆不同。當使用的固定酵素薄膜厚度為40微米時, 在0.01M磷酸緩衝液中, 電極之穩態應答與盤林西林的濃度在1到12mM之間成正比。應答時間約1分鐘。提高緩衝液的離子強度到0.025M, 可提高定量的線性範圍到16mM, 但靈敏度會隨著下降。增加薄膜厚度會增長應答時間, 並且減低靈敏度。重覆檢測實驗結果證實所製備之電極有高度重現性。
• 英文摘要： A penicillin electrode was constructed by coupling a pH electrode and a penicillinase-immobilized membrane prepared by entrapping enzyme within gluten matrix. The use of gluten takes many advantages because it is biodegradable, harmless to the environment, cheaper and readily available. This penicillin electrode showed different responses to penicillin G solution, depending on the buffer strength , thickness of membrane and penicillin concentration. When the enzyme-immobilized membrane with a thickness of 40.mu.m was used, the steady-state response to increasing concentration of penicillin G buffered with 0.01 M phosphate was linear in the range of 1 to 12 mM. The response time was about 1 min. Employing a higher buffer strength (0.025 M phosphate) could increase the upper limit of the linear calibration of penicillin to 16 mM, accompanying with the compensation of a lower sensitivity. Increasing the thickness of membrane increased the response time and reduced the sensitivity. Highly reproducibility was evident from the repeated measurements.
• 中文關鍵字： 盤尼西林電極; 麩蛋白; 固定盤尼西林; 包埋法; 生物電極
• 英文關鍵字： Penicillin Electrode; Gluten; Immobilized Penicillinase; Entrapment; Bioelectrode