- 作者:
- 作者服務機構: Department of Life Science and Engineering, Harbin Institute of Technology (HIT),Harbin, Mainland China
- 中文摘要: --
- 英文摘要:
Background: Rab GTPases function as modulators in intracellular transport. Rab5a, a member of
the Rab subfamily of small GTPases, is an important regulator of vesicle traffic from
the plasma membrane to early endosomes. Recent findings have reported that Rab5a
gene was involved in the progression of cancer. In the present study, we investigated
the effect of Rab5a on cervical cancer invasion and metastasis and the molecular
mechanism underlying the involvement of Rab5a.
Methods: Rab5a expression was assessed by immunohistochemical analysis on a cervical cancer
tissue microarray. RNA interference (RNAi) was performed to knock down the
endogenous expression of Rab5a gene in HeLa and SiHa cells. Cell motility was
evaluated using invasion assay and wound migration assay in vitro. The expression
levels of integrin-associated molecules were detected by Western blot and
immunofluorescence.
Results: We found that Rab5a was expressed at a high level in cervical cancer tissues.
Silencing of Rab5a expression significantly decreased cancer cell motility and
invasiveness. The down-regulation of integrin-associated focal adhesion signaling
molecules was further detected in Rab5a knockdown cells. Meanwhile, active
GTP-bound Rac1, Cdc42, and RhoA were also down-regulated, accompanied with the
reduction in the number and size of filopodia and lamellipodia.
Conclusions: Taken together, these data suggest that Rab5a functions in regulating the invasion phenotype, and we propose that this regulation may be via integrin-mediated signaling pathway in cervical cancer cells.
- 中文關鍵字: --
- 英文關鍵字: --