- 作者: 衛偉,高守國,石南寧
- 作者服務機構: The School of Public Health, Southeast University, Nan Jing, 210019, P. R. China
- 中文摘要: The interaction between gatifloxacin mesylate (GM) and salmon sperm DNA was studied by fluorescence spectrometry and ultra-violet (UV) spectrometry. Additions of salmon sperm DNA to GM solution resulted in its strong fluorescence quenching and UV absorbance decrease due to the strong interaction between GM and salmon sperm DNA. Both the maximum fluorescence emission wavelength and the maximum UV absorbance wavelength of GM did not change with the increasing concentration of salmon sperm DNA, indicating no intercalative binding existed between them. The Stern-Volmer plot indicated that the fluorescence-quenching constant at different temperatures or different salmon sperm DNA concentration ranges was different. Effects of ionic strength and I- on the fluorescence quenching of GM by salmon sperm DNA indicated that electrostatic interaction and groove binding coexisted between them.
- 英文摘要: --
- 中文關鍵字: Salmon sperm DNA; Gatifloxacin mesylate; Interaction; Fluorescence quenching.
- 英文關鍵字: --