- 作者: Hsing-Hui Wu; Tzu-Yu Hsiao; Chiang-Ting Chien; Ming-Kuen Lai
- 作者服務機構: Department of Medicine, Kuang-Tien General Hospital, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Prolonged ischemia amplified iscehemia/reperfusion (IR) induced renal apoptosis and
autophagy. We hypothesize that ischemic conditioning (IC) by a briefly intermittent
reperfusion during a prolonged ischemic phase may ameliorate IR induced renal dysfunction.
We evaluated the antioxidant/oxidant mechanism, autophagy and apoptosis in the
uninephrectomized Wistar rats subjected to sham control, 4 stages of 15-min IC (I15·4), 2
stages of 30-min IC (I30·2), and total 60-min ischema (I60) in the kidney followed by 4 or 24
hours of reperfusion. By use of ATP assay, monitoring O2
-. amounts, autophagy and apoptosis
analysis of rat kidneys, I60 followed by 4 hours of reperfusion decreased renal ATP and
enhanced reactive oxygen species (ROS) level and proapoptotic and autophagic mechanisms,
including enhanced Bax/Bcl-2 ratio, cytochrome C release, active caspase 3,
poly-(ADP-ribose)-polymerase (PARP) degradation fragments, microtubule-associated
protein light chain 3 (LC3) and Beclin-1 expression and subsequently tubular apoptosis and
autophagy associated with elevated blood urea nitrogen and creatinine level. I30·2, not I15·4
decreased ROS production and cytochrome C release, increased Manganese superoxide
dismutase (MnSOD), Copper-Zn superoxide dismutase (CuZnSOD) and catalase expression
and provided a more efficient protection than I60 against IR induced tubular apoptosis and
autophagy and blood urea nitrogen and creatinine level. We conclude that 60-min renal
ischemia enhanced renal tubular oxidative stress, proapoptosis and autophagy in the rat
kidneys. Two stages of 30-min ischemia with 3-min reperfusion significantly preserved renal
ATP content, increased antioxidant defense mechanisms and decreased ischemia/reperfusion
enhanced renal tubular oxidative stress, cytosolic cytochrome C release, proapoptosis and
autophagy in rat kidneys. - 中文關鍵字: --
- 英文關鍵字: --