- 作者: 陳芸; 賴鴻緒; 陳維昭
- 作者服務機構: 國立台灣大學醫學院附設醫院外科部
- 中文摘要: Carnitine在肝臟部分切除後肝細胞再生過程所扮演的角色一直未定。本研究的目的是分析大鼠肝臟部分切除後,血清及剩餘肝臟內Carnitine濃度的變遷。以重約200公克Wistar雄性大鼠40隻切除其肝臟之中間葉及左側葉(約占67%),另以40隻作剖腹假性手術為對照組。手術前4小時開始禁食至術後2小時。在術後第6、24、48、72小時各殺死10隻犧牲採樣,測量其血清Carnitine濃度、剩餘肝臟內Carnitine及血清游離脂肪酸及其代謝物的濃度。結果發現:(1)血清Carnitine濃度於切肝術後6小時即開始上升;(2)剩餘肝臟內Carnitine含量亦於切肝術後6小時開始增加;(3)切肝術後6小時可發現血清游離脂肪酸上升及酮類比值明顯下降。本研究結果顯示,游離脂肪酸是切肝術後早期肝綑胞再生主要能源物質之一,至於後期脂肪酸之利用率下降,應不是因為Carnitine不足,而是有其他原因所造成。
- 英文摘要: The role of carnitine in the early phase of liver regeneration after partial hepatectomy has been acontroversial issue. The purpose for this study was to evaluate the alterations of remnant liver carnitineand serum carnitine concentrations in partial-hepatectomized rats. Partial hepatectomy, with resection ofthe median and left lateral lobes (67%), was done in 40 male Wistar rats weighing about 200 g. Anothergroup of 40 rats underwent a sham-operation for comparison. All the rats underwent fasting for 4 hoursbefore surgical procedure and 2 hours after surgery. They were killed at 6, 24, 48 or 72h after the opera-tion, ten rats for each time. The serum carnitine concentration, remnant liver carnitine content, serum freefatty acid (FFA), acetoacetate (AA) and beta-hydroxybutyrate (BB) concentrations were measured. It wasfound that: (1) the serum carnitine concentration increased in the early posthepatectomized stage; (2) thecarnitine content in the remnant liver increased in the early posthepatectomized stage; and (3) the serumFFA and its metabolites markedly increased with a depressed ketone body ratio noted in the early post-hepatectomized stage. These results supported the theory that regenerating liver utilized free fatty acidsas an immediate main substrate. The utilization then decreased. Because of a marked increase of thecarnitine concentration in the remnant liver and serum, it is suggested that the carnitine contents were suf-ficient for liver regeneration. There must be other reasons for the decreased utilization of free fatty acids.
- 中文關鍵字: partial hepatectomyp; arnitine.
- 英文關鍵字: --