• 作者： Chen, Dong-Hwang; Wang, Sung-Shyong; Huang, Ting-Chia
• 中文摘要： 本研究發展一結合固定化青黴素醯胺(IPA)與電透析之反應分離系統,藉由青黴素G(Pen- G)的水解來產生6-胺基青黴烷酸(6-APA)。文中對 IPA催化Pen-G水解之動力學,苯基醋酸(PAA)、6-APA與Pen-G之電透析分離,以及Pen-G在含IPA電透析槽中水解之反應分離現象均有探討。由IPA催化 Pen-G水解之動力學研究,得知其產物抑制機構與速率方程式。由PAA、6-APA與Pen-G之電透析研究,得知三者之分離行為與其分子大小及對陰離子交換膜之親和性有密切的關聯。傳送速率最快者為PAA,最慢者為Pen-G,且Pen-G在鄰近陰離子交換膜側發生強烈之濃度極化現象。隨著Pen-G濃度之增加,PAA對6-APA之選擇性提高,但PAA與6-APA之傳送速率降低; 電場強度之增加有助於PAA與6-APA傳送速率之提升,但PAA對6-APA之選擇性減小。由 Pen-G在含IPA電透析槽中水解之研究,得知電透析之利用,有益於IPA催化Pen-G水解效率之提升。
• 英文摘要： A reactor-separator combining immobilized penicillin acylase (IPA) and electrodialysis was developed for the production of .epsilon.-aminopenicillanic acid (.epsilon.- APA) through the enzymatic hydrolysis of penicillin G (Pen- G). The kinetics of Pen-G hydrolysis by IPA, the separation of phenylacetic acid (PAA), 6-APA and Pen-G with electrodialysis, as well as the reaction-separation phenomena of Pen-G hydrolysis in the electrodialyzer with IPA were studied. The products inhibition mechanism and rate equation of Pen-G hydrolysis by IPA were obtained. The transport and separation of PAA, 6-APA and Pen-G in electrodialysis were found to be dependent on their ionic sizes and affinity toward the anion exchange membrane. The transport rates of three ions decreased in the sequence PAA>6-APA>Pen-G. Serious concentration polarization of Pen- G was observed in the vicinity of anion exchange membrane. The increase of Pen-G concentration raised the selectivity factor of PAA to 6-APA but reduced both the transport rates of PAA and 6-APA. The increase of electric field strength raised both the transport rates of PAA and 6-APA, but reduced the selectivity of PAA to 6-APA. The utilization of electrodialysis was found to be helpful for enhancing the efficiency of Pen-G hydrolysis by IPA.
• 中文關鍵字： 青黴素G; 青黴素醯胺; 電透析; 反應; 分離; 水解; 動力學
• 英文關鍵字： Penicillin G; Penicillin Acylase; Electrodialysis; Reaction; Separation; Hydrolysis; Kinetics