• 作者： 于寧; 尹福秀; 姚慧霞; 康金玉
• 作者服務機構： 工業技術研究院聯合工業研究所
• 中文摘要： 澱粉糖化酵素，可以戊二醛為架橋劑而固定在幾丁質及其衍生物上，固定後之幾丁質—澱粉糖化酵素之最適pH值為4.0，最適作用溫度為50℃，km值為1.3×10-1 g∕liter。固定後之酵素，經儲存60天後，仍保有65%之活性。 以幾丁質之去乙醯基衍生物－chitosan為固體支柱物時，活性為使用幾丁質時之三倍，最適作用溫度為65℃。以chitosan－澱粉糖化酵素做可溶性澱粉轉化反應時，30℃時穩定性較好且圓柱反應器（column type）較分批式（Bateh type）為佳。以圓柱反應器經過100小時連續作用後仍保有60%為之糖化率。
• 英文摘要： Glucoamylase could be immobilized on chi-tin and its derivatives-chitosan and N-acetyl-chitosan gel by covalent attachment followedby crosslinking with glutaraldehyde. The op-timum pH, optimum temperature and Km ofchitin-glucoamylase were 4.0, 5.0℃ and 1.3x10-1 g/liter, respectively. It retained 65% ofits activity after storage in a refrigerator forsixty days. Chitosan was a better support for glu-coamylase than chitin. The specific activity ofchitosan-glucoamylase was three times-more thanchitin-glucoamylase and the former had an op-timum temperature of 65℃. Chitosan-gluco-amylase was more stable at an operating tem-perature of 30℃ than at 50℃ in the conversionof soluble starch to glucose. Two types ofreactors had been tested and the result showedthat column type was more convenient andeffective than the batch type reactor. In theoperation of column type reactor, the immobi-lized glucoamylase could retain 60% saccharifica-tion after being used for over one hundred hours.
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