- 作者: Kuang-Wen Liao; Yu-Wen Chang; Steve R. Roffler
- 作者服務機構: Institute of Biomedical Sciences, Academia Sinica, and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: We found multiple cloning site sequences in the reporteduntranslated regions (UTR) of several genes in Genbank.The erroneous information can result in the failure toamplify DNA fragments containing untranslated regionsby RT-PCR. It is suggested that a BLAST search be per-formed when primers are designed for PCR amplificationof the 5' or 3' UTR of genes to ensure that the reportedUTR does not contain plasmid-derived sequences.
- 中文關鍵字: --
- 英文關鍵字: --