- 作者: 邱式鴻
- 作者服務機構: 台灣大學生化科學研究所
- 中文摘要: This account describes work in our laboratory on lens crystallins during the past decade. The major classes of crystallins are reviewed. A systematic and detailed analysis of the kinetic properties of two presumably structural proteins with stable enzymatic activities revealed subtle differences in kinetic mechanisms of these enzyme crystallins and their corresponding cellular enzymes Bioorganic synthesis based on .epsilon.- and.delta.-crystallins isolated from avian and reptilian lenses, two crystallins possessing lactate dehydrogenase and argininosuccinate lyase respectively, provides an efficient and practical tool for enantioselective synthesis of valuable organic intermediates. Understanding the crystallin diversity at the molecular and cellular levels constitutes a major goal of crystallin research, as it provides the framework to unravel the complex process underlying the evolution of lens crystallins in general and may provide insight into their biological significance.
- 英文摘要: --
- 中文關鍵字: Lens Crystallina; Lactate Dehydrogenase; Argininosuccinate Lyase; Enzyme Kinetics; Crystallin; Biological Activity; Evolution
- 英文關鍵字: 晶狀體;乳酸去氫;精氨琥珀酸裂解;酵素動力學;晶體蛋白;生物活性;進化