• 作者： 朱廣邦; 劉立人; 劉秀洲; 鄭登貴
• 作者服務機構： 台北榮民總醫院醫學研究部基因重組室; 國立台灣大學畜產研究所
• 中文摘要： 本文報告本研究室培育出來之第十六及十八型人類孔突瘤病毒(HPV)基因轉殖小白鼠之分析，發現在這些小白
  鼠的各種器官及組織中，只有極微量之HPV轉錄本可被反轉錄?及PCR法所偵測。經過核酸分子甲基化之分析後發現
  HPV轉殖基因上有超級甲基化的現象，可能解釋為何轉殖基因小白鼠未能表現HPV基因之原因。
• 英文摘要： We have previously reported an observation that the regulatory sequence of the human papillomavirus(HPV) type 18 genome elicits only basal and unregulated transcriptional activities in transgenic mice [Chooet al., Virology (1992) 188:378-383]. We have now extended the study to other transgenic mice carryingan entire or a subgenomic fragment of HPV-16 and-18. Extremely low levels of HPV transcripts weredetected in various organs of the mice only by reverse transcriptase-based polymerase chain reaction. Onanalysis of the methylation status of the HPV transgenome sequences, hypermethylation was clearly ob-served, therefore suggesting a possible explanation for low level HPV trangenome transcriptional activityin HPV transgenic mice.
• 中文關鍵字： human papillomavirus; transgenic mice; hypermethylation
• 英文關鍵字： --