- 作者: D R Suresh; Vamseedhar Annam; K Pratibha; B V Maruti Prasad
- 作者服務機構: Department of Biochemistry, Sri Siddhartha Medical College, SSAHE, Tumkur, India
- 中文摘要: --
- 英文摘要:
Background :
Oxidative stress induced by the production of reactive oxygen species may play a critical role in the stimulation of HIV replication and the development of
immunodeficiency. This study was conducted as there are limited and inconclusive
studies on the significance of a novel early marker of oxidative stress which can
reflect the total antioxidant capacity in HIV patients,
Methods :
Total antioxidant capacity (TAC) and lipid peroxidation were evaluated in 50 HIV-1
seropositive patients (including HIV-1 symptomatics and asymptomatics). Controls
included 50 age and sex matched and apparently healthy HIV-1 seronegative subjects.
Serum malondialdehyde (MDA), Total antioxidant capacity[TAC] (by ferric reducing
antioxidant power assay), vitamin E, vitamin C and superoxide dismutase (SOD)
enzyme activity were estimated among controls and cases. Statistical comparisons and
correlations at 5% level of significance were determined.
Results and Discussion
The mean MDA concentrations were significantly elevated in both HIV-1
asymptomatic (CD4+ count > 500 cells/microliter) and HIV-1 symptomatic (CD4+
count <500 cells/microliter) groups (Mean ± S.D values were 2.2 +/- 0.7 nmol/ml and
2.8 +/- 0.8 nmol/ml respectively) when compared with the control group (Mean ± S.D
value was 0.9 +/- 0.2 nmol/ml) (p<0.01). The mean TAC of HIV- 1 asymptomatic and
HIV-1 symptomatic (Mean ± S.D values were 754.6±135.6 μmol/L and 676.6±154.1
μmol/L respectively) patients were significantly reduced compared with the control
group (Mean ± S.D value was 1018.7±125.6 μmol/L) (p<0.01). Also, there were
significantly decreased levels of vitamin E, vitamin C and SOD among HIV-1
seropositive patients(controls > asymptomatic > symptomatic) compared to controls
3
(p<0.01). TAC showed significant negative correlation with MDA among HIV-1
infected patients (p<0.01).
Conclusion :
Our results clearly show that severe oxidative stress occurs in the HIV-1 seropositive
patients in comparison with controls, and increases significantly with the progression
of disease, i.e. HIV-1 symptomatics > asymptomatics > controls. TAC can be used as
a novel early bio-chemical marker of oxidative stress in HIV-1 infected patients
which may result in reduced tissue damage by free radicals and help to monitor and
optimize antioxidant therapy in such patients. - 中文關鍵字: --
- 英文關鍵字: --