- 作者: Shu-Ying Chen; George Hsiao; Hwong-Ru Hwang; Pao-Yun Cheng; Yen-Mei Lee
- 作者服務機構: 1 Department of Nursing, HungKuang University, Taichung, Taiwan; ; 2 Department of Pharmacology, Taipei Medical University, Taipei, Taiwan; ; 3 Division of Cardiology, Department of Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; ; 4 Department of Pharmacology, National Defense Medical Center, No. 161, Section 6, Min-Chuan East Road, NeiHu 114, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: The accumulation of oxygen free radicals and activation of neutrophils are strongly implicated as pathophysiological mechanisms mediating myocardial ischemia/reperfusion injury. Heme oxygenase-1 (HO-1) has been reported to play a protective role in oxidative tissue injuries. In this study, the cardioprotective activity of tetramethylpyrazine (TMP), an active ingredient of Chinese medicinal herb Ligusticum wallichii Franchat, was evaluated in an open-chest anesthetized rat model of myocardial ischemia/reperfusion injury. Pretreatment with TMP (5 and 10 mg/kg, i.v.) before left coronary artery occlusion significantly suppressed the occurrence of ventricular fibrillation. After 45 min of ischemia and 1 h of reperfusion, TMP (5 and 10 mg/kg) caused a significant reduction in infarct size and induced HO-1 expression in ischemic myocardium. The HO inhibitor ZnPP (50 μg/rat) markedly reversed the anti-infarct action of TMP. Superoxide anion production in ischemic myocardium after 10 min reperfusion was inhibited by TMP. Furthermore, TMP (200 and 500 μΜ) significantly suppressed fMLP (800 nM)-activated human neutrophil migration and respiratory burst. In conclusion, TMP suppresses ischemia-induced ventricular arrhythmias and reduces the infarct size resulting from ischemia/reperfusion injury in vivo. This cardioprotective activity of TMP may be associated with its antioxidant activity via induction of HO-1 and with its capacity for neutrophil inhibition.
- 中文關鍵字: --
- 英文關鍵字: arrhythmias, HO-1, myocardial ischemia, neutrophils, oxidative stress, reperfusion injury, tetramethylpyrazine