• 作者： Ng, I-Son; Tsai, Shau-Wei; Chen, Shu-Jen
• 中文摘要： 自Acinetobacter radioresistens CMC-2可生產具有耐熱性以及耐溫性之菌體外脂肪酵素。經過離心、超過濾及冷凍真空操作, 可回收49.5%總活性之粗酵素, 再經過進一步之親油性管柱層析及超過濾操作, 可回收26.6%總活性之純化酵素。以SDS-PAGE及IEF分析, 獲得脂肪酵素之分子量及等電點分別為38kDa與4.5。文中除了探討pH值、溫度、金屬離子、界面活性劑及有機溶劑對酵素活性與穩定性影響外, 並發現本酵素對三酸甘油酯具1,3位置選擇性, 以及對外消旋 suprofen三氟乙基酯具(R)-形立體異構物選擇性。
• 英文摘要： A novel lipase with the favorable alkaline and thermostable characteristics was produced from Acinetobacter radioresistens CMC-2. The crude lipase with 49.5% of total activity was recovered after centrifugation, ultrafiltration and lyophilization. The crude preparation was further purified to a homogeneous state by column chromatography on phenyl sepharose and ultrafiltration, giving 26.6% of total activity recovery. The molecular weight and isoelectric point of the lipase determined by SDS-PAGE and IEF were 38kDa and 4.5, respectively. The lipase could be classified as a 1,3-positional specific enzyme and possessed the(R)-stereoselectivity in the hydrolysis of racemic suprofen trifluoroethyl ester in isooctane. Moreover, effects of pH, temperature, metal ions, surfactants and organic solvents on the enzyme activity and stability were reported.
• 中文關鍵字： 鹼性脂肪酵素; 純化; 物性分析
• 英文關鍵字： Alkaline Lipase; Purification; Characterization Analysis