- 作者: Ian G. Macreadie; Ross Fernley; Laura A. Castelli; Anna Lucantoni; Jacinta White; A. Azad
- 作者服務機構: a Biomolecular Research Institute and ; b CSIRO Molecular Science, Parkville, Vic, Australia
- 中文摘要: --
- 英文摘要: The human immunodeficiency virus type 1 (HIV-1) Nef protein is essential for AIDS pathogenesis, but its function remains highly controversial. During stresses such as growth in the presence of copper or at elevated temperature, myristylated Nef is released from yeast cells and, after extended culture in stationary phase, it accumulates in the supernatant as a dense membranous material that can be centrifuged into a discrete layer above the cell pellet. This material is unique to Nef-producing cells and represents a convenient source of Nef that may have application in further biological studies. Within the yeast cell, electron microscopic examination shows that Nef localises in novel, membrane-bound bodies. These data support the evidence for a role of Nef in membrane perturbation and suggest that there may be a similar localisation for myristylated Nef in HIV-1 infected cells.
- 中文關鍵字: --
- 英文關鍵字: Acquired immunodeficiency syndrome ; Human immunodeficiency virus ; Nef protein ; Myristylation; Membrane permeabilisation ; Saccharomyces cerevisiae ; Yeast