- 作者: Yan Huang, Zhong-Quan Dai; Shu-Kuan Ling; Hong-Yu Zhang; Yu-Min Wan; Ying-Hui Li
- 作者服務機構: State Key Laboratory of Space medicine Fundamentation and Application, China Astronaut Research and Training Center, Beijing, China
- 中文摘要: --
- 英文摘要:
Background: Stem cell therapy has emerged as a potential therapeutic option for tissue
engineering and regenerative medicine, but many issues remain to be resolved, such as the amount
of seed cells, committed differentiation and the efficiency. Several previous studies have focused on
the study of chemical inducement microenvironments. In the present study, we investigated the
effects of gravity on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into
force-sensitive or force-insensitive cells.
Methods and results: Rat BMSCs (rBMSCs) were cultured under hypergravity or simulated
microgravity (SMG) conditions with or without inducement medium. The expression levels of the
characteristic proteins were measured and analyzed using immunocytochemical, RT-PCR and
Western-blot analyses. After treatment with 5-azacytidine and hypergravity, rBMSCs expressed
more characteristic proteins of cardiomyocytes such as cTnT, GATA4 and -MHC; however,
fewer such proteins were seen with SMG. After treating rBMSCs with osteogenic inducer and
hypergravity, there were marked increases in the expression levels of ColIA1, Cbfa1 and ALP.
Reverse results were obtained with SMG. rBMSCs treated with adipogenic inducer and SMG
expressed greater levels of PPARgamma. Greater levels of Cbfa1- or cTnT-positive cells were
observed under hypergravity without inducer, as shown by FACS analysis. These results indicate
that hypergravity induces differentiation of rBMSCs into force-sensitive cells (cardiomyocytes and
osteoblasts), whereas SMG induces force-insensitive cells (adipocytes).
Conclusion: Taken together, we conclude that gravity is an important factor affecting the
differentiation of rBMSCs; this provides a new avenue for mechanistic studies of stem cell
differentiation and a new approach to obtain more committed differentiated or undifferentiated
cells. - 中文關鍵字: --
- 英文關鍵字: --