- 作者: 眭台生 ; 莊美英
- 作者服務機構: 中山大學化學系
- 中文摘要: Simultaneous determination of cysteine (RSH) and cystine (RSSR), two important sulfur-containing amino acids, in capillary electrophoresis(CE) has been an analytical task. Dual-microelectrode amperometric detection seems to be a good scheme, but significant difficulty in electrode construction and poor detection limit for RSSR determination remain major short-comings. In reverse pulse amperometric (RPA) detection, the applied potentials are repeatedly pulsed back and forth between the reducing initial potential (e.g., E/sub i/ =-1.4 V) and the oxidizing final potential (e.g., E/sub f/ = 0.0 V) at a single, gold-mercury amalgam (Au/Hg) microelectrode. At E/sub i/, RSSR is reduced to RSH which causes catalytical oxidation of the Au/ Hg amalgam microelectrode when the potential is pulsed to E/sub f/. The resulting anodic current is then recorded. Therefore, by using RPA detection after CE separation, RSH and RSSR can be simultaneously determined.
- 英文摘要: --
- 中文關鍵字: Capillary Electrophoresis (Ce); Reverse Pulse; Pulsed Amperometric Detection; Mercury Film Electrode; Au/Hg Electrode; Cysteine
- 英文關鍵字: 毛細管電泳;逆脈衝;脈衝電流偵測;汞薄膜電極;銀汞電極;半胱胺酸