- 作者: Yi-Wen Lin; Hsiu-Wen Yang; Ming-Yuan Min; Tsai-Hsien Chiu
- 作者服務機構: 1 Institute of Biomedical Science, Academia S璯ica, Taipei, 115, Taiwan; ; 2 Department of Life Sciences, Chung Shan Medical University, Taichung, 402, Taiwan;; 3 Department of Life Science, National Taiwan University, Taipei, 106, Taiwan; ; 4 Department of Physiology, National Yang Ming University, Taipei, 102, Taiwan
- 中文摘要: --
- 英文摘要: The aim of this study was to investigate the role of β-adrenergic receptors in modulating associative long-term depression (LTD) at CA1 synapses in rat hippocampal slices. Standard extracellular electrophysiological techniques were employed to record field excitatory post-synaptic potential (fEPSP) activity and to induce associative LTD. Two independent Schaffer collateral pathways were elicited in hippocampal CA1 areas. In one (weak) pathway, the stimulating intensity was adjusted to elicit small fEPSP activity (20-30% of the maximum response). In contrast, 80-90% of the maximum response was evoked in the other (strong) pathway. Associative LTD of weak pathway could be induced by paired stimulation of weak and the strong pathways, repeated 100 times at 0.167 Hz. The associative LTD of weak pathway was NMDA receptor- and phophatase 2B dependent, because bath application of 50 μΜ D, L-AP5 or 10 μΜ cypermethrin blocked its induction. Bath application of 1 μΜ isoproterenol inhibited associative LTD, and this effect was blocked by timolol, suggesting the involvement of β-adrenergic receptors. The inhibitory effect of β-adrenergic receptors on LTD induction was blocked in slices pretreated with inhibitors of protein kinase A and mitogen-activated protein kinase, suggesting that these signal cascades are downstream effectors following activation of β-adrenergic receptors. Nevertheless, bath application of timolol or cypermethrin alone did not have significant effect on associative LTD induction, suggesting neither endogenous function of β-adrenergic receptor nor endogenous PKA activity does have a role in associative LTD induction.
- 中文關鍵字: --
- 英文關鍵字: associative LTD, β-adrenergic receptors, hippocampus