- 作者: 吳龍溪
- 作者服務機構: 國立臺灣大學
- 中文摘要:
綠豆受苗立枯病菌(Rhizoctonia solani)感染後,在發病初期,還元醣及氨基酸含量激增,
核酸及蛋白質含量?減少,尤以子葉所含水溶性蛋白質為甚,並且蛋白質電泳?式亦改變,其代謝
作用顯然被病原菌擾亂。
本試驗為探求罹病苗代謝作用轉變之原因,先測碳水化合物代謝有關酵素5種,其結果另與報
告,今為進一步瞭解其?象,測8種脫氫?(Dehydrogenase)之活性,發現綠豆在接種後24小時
內,其活性比健苗大致增高,但接種72小時以後,則病斑出現,其活性突降,比健苗差,經比較罹
病苗與病原菌之脫氫?活性,得知罹病苗脫氫?活性之變化,純是罹病效應所致,並不參與病原菌
脫氫?之活性在內。其中2種脫氫?(琥珀酸脫氫?及蘋果酸脫氫?),對綠豆病態發展具有重要
角色。
y - 英文摘要: Assays of the dehydrogenase were based on the amount of formazan formedfrom the reduction of 3-p-iodophenyl-2-2-p-nitrophenyltetrazolium chloride (INT)through the intermediate electron carrier, phenazine methosulfate (PMS), for thetransfer of electron from either reduced enzyme or coenzyme to the tetrazoliumsalt. Rhizoctonia infection was shown to cause the dehydrogenase activity of theinoculated plant to rise significantly above that of the healthy plant within the first24 hours though no visible symptoms appeared. However, as soon as the symptomsappeared, i.e., 72 hours after inoculation, the dehydrogenase activity was declinedmore rapidly in the infected plants. The alteration of enzyme activity in the hostplant was not directly associated with dehydrogenases of fungal origin. Among theenzyme tested, succinate dehydrogenase and malate dehydrogenase might be thegoverning enzymes in response of parasitic attack.
- 中文關鍵字: --
- 英文關鍵字: --