- 作者: Chia-Ying Chuang; Chin Hsu; Che-Yi Chao; Yung-Shung Wein; Yueh-Hsiung Kuo; Ching-jang Huang
- 作者服務機構: 1 Nutritional Biochemistry Laboratory, Institute of Microbiology and Biochemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Tapei, 106, Taiwan; ; 2 Department of Applied Life Science, Asia University, 500 Lioufeng Road, Wufong Township, Taichung County, 413, Taiwan; ; 3 Department of Chemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei, 106, Taiwan; ; 4 Department of Biochemical Science and Technology, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei, 106. Taiwan; ; 5 Institute of Bio-Agricultural Science, Academia S璯ica, Taipei, 115, Taiwan
- 中文摘要: --
- 英文摘要: Bitter gourd (Momordica charantia L.) is a common vegetable in Asia that has been used in traditional medicine for the treatment of Diabetes. PPARs are ligand-dependent transcription factors that belong to the steroid hormone nuclear receptor family and control lipid and glucose homeostasis in the body. We previously reported that the ethyl acetate (EA) extract of bitter gourd activated peroxisome proliferator receptors (PPARs) α and γ. To identify the active compound that activated PPARα, wild bitter gourd EA extract was partitioned between n-hexane and 90% methanol/10% H2O, and the n-hexane soluble fraction was further separated by silica gel column chromatography and finally by preparative HPLC A transactivation assay employing a clone of CHOKl cells stably transfected with a (UAS)4-tk-alkaline phosphatase reporter and a chimeric receptor of GAL4-rPPARα LBD was used to track the active component. Based on Mass. NMR, and IR spectroscopy, 9cis, 11 trans, 13tran.s-conjugated linolenic acid (9c, 11t, 13t-CLN) was identified as a PPARα activator in wild bitter gourd. The isolated 9c, 11t, 13t-CLN rich fraction also significantly induced acyl CoA oxidase (ACO) activity in a peroxisome prolif-erator-responsive murine hepatoma cell line, H4IIEC3, implying that 9c, 11t, 13t-CLN was able to act on a natural PPARα signaling pathway as well. The content of 9c, 11t, 13t-CLN was estimated to be about 7.1 g/kg of our dried wild bitter gourd sample. The concentration of 9c, llt, 13t-CLN and activation activity in the hydrolyzed EA extract of the seeds was higher than that of the flesh. The potential health benefits of 9c, llt, 13t-CLN through the PPARα regulated mechanism are worthy to be further characterized in in vivo studies.
- 中文關鍵字: --
- 英文關鍵字: PPARα, 9cis, 11trans, 13trans-conjugated linolenic acid, wild bitter gourd, transactivation assay, Acyl CoA Oxidase