- 作者: Fan-Ching Shen, Ih-Jen Su, Han-Chieh Wu, Yi-Hsuan Hsieh, Wei-Jen Yao, Kung-Chia Young, Tsung-Chuan Chang, Hui-Chuan Hsieh, Han-Ni Tsai, Wenya Huang
- 作者服務機構: Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background :
Chronic hepatitis B virus (HBV) infection is an important cause of hepatocellular carcinoma
(HCC) worldwide. The pre-S1 and -S2 mutant large HBV surface antigen (LHBS), in which
the pre-S1 and -S2 regions of the LHBS gene are partially deleted, are highly associated with
HBV-related HCC.
Methods :
The pre-S region of the LHBS gene in two hundred and one HBV-positive serum samples
was PCR-amplified and sequenced. A pre-S oligonucleotide gene chip was developed to
efficiently detect pre-S deletions in chronic HBV carriers. Twenty serum samples from
chronic HBV carriers were analyzed using the chip.
Results :
The pre-S deletion rates were relatively low (7%) in the sera of patients with acute HBV
infection. They gradually increased in periods of persistent HBV infection: pre-S mutation
rates were 37% in chronic HBV carriers, and as high as 60% in HCC patients. The Pre-S
Gene Chip offers a highly sensitive and specific method for pre-S deletion detection and is
less expensive and more efficient (turnaround time 3 days) than DNA sequencing analysis.
Conclusions :
The pre-S1/2 mutants may emerge during the long-term persistence of the HBV genome in
carriers and facilitate HCC development. Combined detection of pre-S mutations, other
markers of HBV replication, and viral titers, offers a reliable predictive method for HCC risks
in chronic HBV carriers. - 中文關鍵字: --
- 英文關鍵字: --