- 作者: Wen-Chang Chang; Wei-Juin Shyu; Guey-Yueh Shi; Ming T. Lin; Chauyin J. Jen; Lih-Yuh C. Wing ; Ming-Jer Tang; Hua-Lin Wu
- 作者服務機構: Departments of; a Pharmacology, ; b Biochemistry and ; c Physiology College of Medicine,National Cheng Kung University, Tainan, Taiwan, Republic of China
- 中文摘要: --
- 英文摘要: Treatment of cultured bovine carotid artery endothelial cells with 0.1 μM human plasmin has been reported to induce a receptor-mediated short burst of arachidonate release, which is a pertussis toxin-sensitive and extracellular calcium-dependent reaction. Plasmin-induced calcium influx in cells was significantly inhibited by pretreatment with pertussis toxin, indicating that the former was coupled with a pertussis toxin-sensitive guanosine 5'-triphosphate (GTP)-binding protein. Plasmin significantly induced the formation of lyso-phosphatidylcholine but not lysophosphatidylethanolamine. A cellular phospholipase A2 with an arachidonyl specificity at the sn-2 position of phosphatidylcholine, which required submicromolar calcium, was identified as a cytosolic phospholipase A2 by immunoblot analysis. By a cell-free enzyme activity assay and immunoblot analysis, plasmin was found to induce a translocation of the cytosolic phospholipase A2 from the cytosol to the membrane. Taken together, the results suggest that plasmin bound to its putative receptor and activated a GTP-binding protein coupled to calcium influx channel, followed by translocation and activation of cytosolic phospholipase A2 in endothelial cells.
- 中文關鍵字: --
- 英文關鍵字: Plasmin ; Calcium influx Cytosolic phospholipase A2 ; Endothelial cells