- 作者: K. Siddappa,* M. Mallikarjun, Tukaram Reddy and Mahesh Tambe
- 作者服務機構: Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga-585106, Karnataka, India
- 中文摘要: Simple, sensitive, rapid and cost effective extraction spectrophotometric methods are described for the assay of mebeverine hydrochloride (MBH) in bulk samples and pharmaceutical formulations. These two methods (Bromophenol blue and Erichrome Black-T) are based on the formation of chloroform soluble ion-pair complexes of MBH with Bromophenol blue (BPB) and with Erichrome Black-T (EBT), to form yellow and pink colored chromogen in a Glycine-HCl buffer of pH 2.4 (BPB) and in a KCl-HCl buffer of pH 1.4 (EBT) with absorbance maximum at 416 nm and at 524 nm for BPB and EBT respectively. The calibration graph is found to linear over 0.2-20μg/mL (BPB) and 0.2-20μg/mL (EBT), with molar absorptivity values of 1.8295 104 l moL-1 cm-1 and 1.5896 104 l moL-1 cm-1, respectively. The LOD (Limit of Detection) were found to be 0.090μg/mL and 0.084μg/mL and LOQ (Limit of Quantification) were 0.2997μg/mL and 0.2730μg/mL for the BPB and EBT method, respectively. The results of analysis for the two methods have been validated statistically and by recovery studies. The results are compared with those obtained with reported method. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.
- 英文摘要: --
- 中文關鍵字: UV-Visible spectrophotometer; Mebeverine hydrochloride; Bromophenol blue; Erichrome Black-T.
- 英文關鍵字: --