- 作者: 吳啟裕 ; 陳水田 ; 何純郎 等
- 作者服務機構: 中央研究院生物化學研究所
- 中文摘要: Two 60-residue snake toxins with four disulfide bridges, cardiotoxin II and IV, of Taiwan cobra (Naja naja atra) have been rapidly prepared in overall yields of 3.9%(cardiotoxin II) and 3.7% (cardiotoxin IV) within three weeks using the chemical method. Physicochemical characterization of these synthetic cardiotoxins was carried out by amino acid analysis, mass spectroscopy, capillary electrophoresis, peptide mapping, circular dichroism spectroscopy, and lethal toxicity. As compared with natural cardiotoxins, the results indicated that the synthetic cardiotoxins possessed the same physicochemical properties as those of natural ones. Therefore, in addition to preparation of various important toxins with satisfactory quantities for biochemical and pharmacological studies within a short time, this rapid method also provides an important route to obtain many interesting toxins and designed toxin analogues for structure/function relationship studies in the near future.
- 英文摘要: --
- 中文關鍵字: Snake Venom; Taiwan Cobra; Protein Folding; Cardiotoxin
- 英文關鍵字: 蛇毒;眼鏡蛇;蛋白摺疊