- 作者: 黃承文,周阜民
- 作者服務機構: Department of Chemistry, Tunghai University, Taichung 407, Taiwan, R.O.C.
- 中文摘要: A new analytical method for phenylpropanolamine based on micellar electrokinetic chromatographic separation and laser-induced fluorescence detection has been developed. Naphthalene-2,3-dicarboxaldehyde was used for precolumn derivatization of the nonfluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) containing 15 mM sodium dodecyl sulfate and a He-Cd laser (lex: 442 nm, lem: 500 nm). Linearity (r 3 0.99) of two orders of magnitude was generally obtained and the concentration limit of detection was in the ng/mL level. Coupled with a simple cleanup procedure, the method can be applied to the analysis of phenylpropanolamine in human plasma, with a limit of detection at 15 ng/mL. Recovery of phenylpropanolamine from plasma samples was about 90%.
- 英文摘要: --
- 中文關鍵字: Phenylpropanolamine; Micellar electrokinetic chromatography; Laser-induced fluorescence detection; Plasma.
- 英文關鍵字: --