• 作者： 趙有誠; 王明芳; 唐鴻舜; 許重得; 尹士俊
• 作者服務機構： 三軍總醫院內科部骨腸科; 國防醫學院生化學科
• 中文摘要： 此研究我們利用聚合?連鎖反應及限制?切割片段長短之多形性鑑識中國人酒精性肝硬化及不酗酒病人乙醇脫氫
  ?2 (ADH2)及乙醇脫氫?3 (ADH3)之基因型。此方法不具放射線，操作容易且再現性極佳。研究結果顯示在酒精性
  肝硬化病人ADH2* 1及ADH3*2對偶基因出現頻數，有意義的高於病毒性肝硬化及胃或十二指腸潰瘍等對照組病人。相
  對地。病毒性肝硬化患者和胃或十二指腸潰瘍病人間，ADH2及ADH3對偶基因型分佈形態則相似。上述結果暗示
  ADH2*2及ADH3*1對偶基因可能在中國人扮演預防酗酒之保護角色。
  學
• 英文摘要： We investigated the genotype of ADH2 and ADH3 in Chinese alcoholic cirrhotics and non-alcoholicsby using a polymerase chain reaction and restriction-fragment-length polymorphism. This method is non-radioactive, easy to implement with good reproducibility. In the Chinese population, the frequencies ofthe ADH2*1 and ADH3*2 alleles were significantly higher in the alcoholic cirrhotic patients (53%;23%)than in the viral hepatitis cirrhotics (32%;8%) and the gastric and/or duodenal ulcer control patients (25%;6%). On the other hand, the gastric and/or duodenal ulcer control patients and the viral hepatitis cirrhoticpatients showed similar allele frequencies for the polymorphic ADH2 and ADH3 genes. These findingssuggest that the alleles ADH2*2 and ADH3*1, coding for the high-V β-ADH and -ADH, respectively,may play a protective role against alcoholism in Chinese patients.
• 中文關鍵字： alcohol dehydrogenase; genotype; polymerase chain reaction; restriction fragment length poly-morphism; cirrhosis.
• 英文關鍵字： --