- 作者: 王來好,田憲儒,戴俊耀
- 作者服務機構: Department of Applied Chemistry, Chia Nan University of Pharmacy and Science, Tainan 71743, Taiwan, R.O.C.
- 中文摘要: Chemically modified electrodes, constructed by incorporating potassium hexachloroplatinate K2PtCl6 into the graphite power/paraffin oil matrix used to fabricate conventional carbon paste electrodes, were shown to catalyze the electrooxidation of purine compounds including theophylline, 3-methylxanthine, 1,3-dimethyluric acid and 1-methyluric acid; the modifier electrodes display electrocatalytic activity for the oxidation of purines at 170 mV (vs Ag/AgCl) when used as sensing electrodes in amperometric detection following liquid chromatography. The K2PtCl6 electrodes permitted detection of the purines at similar values of applied potential. The limit of detection (LOD) of theophylline, 1-MUA, 3-MXT and 1,3-DMUA were 1.1 ng mL-1, 3.2 ng mL-1, 1.0 ng mL-1 and 11 ng mL-1, respectively. The calibration graphs were linear for theophylline and metabolites over the range of concentration used (50-3200 ng mL-1). Theophylline and its metabolites can easily be separated and analyzed in one run using electrochemical sensor chromatography with a methanol - phosphate buffer containing 20 mM di-potassium hydrogen phosphate (pH 5.50). Serum samples were collected after the oral administration of black tea from human volunteers. Quantitative data for the determination of a micromolar amount of theophylline and its metabolites in serum samples are in agreement with data obtained by HPLC-UV.
- 英文摘要: --
- 中文關鍵字: Electrochemical sensor; Disposable modified carbon paste electrode; Theophylline and three metabolites.
- 英文關鍵字: --