- 作者: Pei-Jiun Tsai, Hwai-Shi Wang, Yi-Ming Shyr, Zen-Chung Weng, Ling-Chen Tai, Jia-Fwu Shyu, Tien-Hua Chen
- 作者服務機構: Institute of Clinical Medicine, National Yang-Ming University, Taipei, Republic of China
- 中文摘要: --
- 英文摘要:
Background: Although diabetes mellitus (DM) can be treated with islet transplantation, a scarcity of donors
limits the utility of this technique. This study investigated whether human mesenchymal stem
cells (MSCs) from umbilical cord could be induced efficiently to differentiate into insulinproducing
cells. Secondly, we evaluated the effect of portal vein transplantation of these
differentiated cells in the treatment of streptozotocin-induced diabetes in rats.
Methods: MSCs from human umbilical cord were induced in three stages to differentiate into insulinproducing
cells and evaluated by immunocytochemistry, reverse transcriptase, and real-time
PCR, and ELISA. Differentiated cells were transplanted into the liver of diabetic rats using a
Port-A catheter via the portal vein. Blood glucose levels were monitored weekly.
Results: Human nuclei and C-peptide were detected in the rat liver by immunohistochemistry.
Pancreatic β-cell development-related genes were expressed in the differentiated cells. Cpeptide
release was increased after glucose challenge in vitro. Furthermore, after
transplantation of differentiated cells into the diabetic rats, blood sugar level decreased.
Insulin-producing cells containing human C-peptide and human nuclei were located in the
liver.
Conclusion: Thus, a Port-A catheter can be used to transplant differentiated insulin-producing cells from
human MSCs into the portal vein to alleviate hyperglycemia among diabetic rats. - 中文關鍵字: --
- 英文關鍵字: Mesenchymal stem cell, Portal vein, Insulin-producing cells, Transplant